Artifacts and artifact removal in optical coherence tomographic angiography
Review Article

Artifacts and artifact removal in optical coherence tomographic angiography

Tristan T. Hormel1, David Huang1, Yali Jia1,2

1Casey Eye Institute, Oregon Health & Science University, Portland, OR, USA; 2Department of Biomedical Engineering, Oregon Health & Science University, Portland, OR, USA

Correspondence to: Yali Jia. Casey Eye Institute, Oregon Health & Science University, Portland, OR, USA. Email:

Abstract: Optical coherence tomographic angiography (OCTA) enables rapid imaging of retinal vasculature in three dimensions. While the technique has provided quantification of healthy vessels as well as pathology in several diseases, it is not unusual for OCTA data to contain artifacts that may influence measurement outcomes or defy image interpretation. In this review, we discuss the sources of several OCTA artifacts—including projection, motion, and signal reduction—as well as strategies for their removal. Artifact compensation can improve the accuracy of OCTA measurements, and the most effective use of the technology will incorporate hardware and software that can perform such correction.

Keywords: Optical coherence tomographic angiography (OCTA); imaging artifacts; artifact correction

Submitted Jun 04, 2020. Accepted for publication Jul 29, 2020.

doi: 10.21037/qims-20-730


Optical coherence tomographic angiography (OCTA) (1-3) provides high-resolution, depth-resolved, non-invasive visualization of vasculature. The technique leverages the fluctuation in signal between consecutive OCT cross-sectional scans caused by flowing blood as an inherent contrast in order to detect vessels down to the capillary scale. While the technique has been applied to image skin (1,4,5), mouse brains (6,7), and developing zebrafish embryos (8), it has so far been most widely used in ophthalmic imaging and eye research (9-11), where it has provided insight into the vascular morphology of healthy eyes (12) and eyes afflicted by vision-threatening diseases such as age-related macular degeneration (AMD) (13-20), diabetic retinopathy (DR) (21-24), glaucoma (25-28), and others (29-34). In this context, researchers have found that image-based measurements commonly made on OCTA, such as vessel density and avascular area, can distinguish between healthy and pathological eyes (22,31,35-37) and sometimes with better accuracy than alternative imaging modalities (38).

While these advantages have led to its rapid adoption as a research tool and in the clinic, as an emerging technology OCTA still suffers some limitations. For example, OCTA fields of view are typically much smaller than those in other angiographic techniques, such as fluorescein angiography (FA) or indocyanine green angiography (ICGA), employed in ophthalmology. This drawback means that pathology that manifests in the peripheral retina may not always be revealed by OCTA. However, the most prominent limitations in OCTA are imaging artifacts. While some artifacts are innocuous or easily detectable, others can be severe while still remaining subtle. Measurement accuracy on OCTA data can be reduced by artifacts, and in the worst cases they can even suggest misleading conclusions. Currently artifacts are common, with one study reporting their presence in 97% of OCTA images (39). It is essential, then, that anyone working with OCTA data understand their effects.

In this review, we will examine the source of OCTA artifacts, including projection, bulk motion, and signal reduction. In order to illuminate the origin of these artifacts we will first discuss signal generation in OCTA.

Flow signal and confounding factors

Flow signal in OCTA is derived from motion contrast between consecutive OCT B-scans. The OCT signal itself is acquired through coherence gating, where interference with a reference arm amplifies the signal from the sample arm in order to resolve the depth at which the signal originates. Contemporary OCT systems replace the original time-domain processing, which required the physical scanning of a reference mirror, with Fourier-domain processing. For illumination from a light source with Gaussian power spectral density, this signal within a line scan at a depth z can be succinctly written (40)

F 1 { I( k ) }( z )=A( z )exp[ iΦ( z ) ] [1]

where F 1 { I( k ) }( z ) denotes the inverse Fourier transform of the detected signal in k-space at depth z, and A(z) and Φ( z ) denote the amplitude and phase of the signal, respectively. As indicated by Eq. [1], motion contrast can manifest in both the phase and amplitude of the complex OCT signal. Consequently, OCTA can be generated by examining motion contrast in the phase, amplitude, or complex signal (41). It is often claimed that phase contrast measurements are more sensitive to slow flow (41,42); however, phase signals need be stabilized or compensated by correcting the bulk motion-generated phase shift before computing OCTA signals. Thus, phase, or complex-signal-based OCTA algorithms are highly dependent on an appropriate phase compensation step (43). Despite these differences, they are largely immaterial in the context of retinal imaging, where flow in capillaries is detectable using either amplitude or phase. Both amplitude and complex-signal-based OCTA approaches can detect very slow flow (including Brownian motion), such as the in vitro intralipid flow when syringe pumps stop (44,45) or the in vivo flow in intraretina cystic spaces (46,47). Most commercial instruments (Optovue, Topcon, and Heidelberg) are amplitude-based, while Zeiss uses complex-signal processing.

In addition to the specifics of the signal being processed to construct OCTA data, the mathematics used to quantify signal variation can also influence the presence of image artifacts. Common approaches include difference, variance, and (de)correlation. To gain an intuition for how the choice of motion contrast metric influences OCTA image generation, we can consider two prominent OCTA construction techniques. In optical microangiography (OMAG) (1), the flow signal is calculated from the complex signal as

I OMAG ( x,z )= [ I n+1 ( x,z ) I n ( x,z ) ] ABS [2]

where In is value of the nth cross-sectional scan at location (x,z), and [ ξ ] ABS indicates magnitude of ξ . In contrast, split-spectrum amplitude decorrelation angiography (SSADA) (2) uses decorrelation instead of difference:

D( x,z )=1 1 N1 1 M n=1 N1 m=1 M A m,n ( x,z ) A m,n+1 ( x,z ) 1 2 A m,n ( x,z ) 2 + 1 2 A m,n+1 ( x,z ) 2 [3]

where An represents the amplitude of the nth cross-sectional scan at (x,z). SSADA also employs the split-spectrum approach wherein the OCT spectrum is calculated across M sub-bands; the decorrelation in each sub-band is averaged to form the final decorrelation value, thereby increasing the signal-to-noise ratio. It is easily seen that D does not scale with the magnitude of A: if Am,n (x,z) and Am,n+1 (x,z)differ by 20%, doubling the value of both will not change the value of D (x,z). On the other hand, if In (x,z) and In+1 (x,z) also differ by 20%, doubling both their values will preserve a 20% ratio, but the value of IOMAG (x,z) will have doubled. This example elucidates the point that in difference (and variance) methods the flow signal is more strongly coupled to the magnitude of the OCT signal than in decorrelation-based methods. This makes correlation/decorrelation approaches less likely to misdetect flow in hyperreflective material. Alternatively, since noise is maximally decorrelated, low signal regions in which noise predominates register a large decorrelation value. For this reason, correlation-based approaches introduce amplitude or phase cutoffs that prevent flow detection in hyporeflective regions. This re-introduces a reflectance magnitude dependence into these approaches.

Projection artifacts

Independent of the specifics of processing, by virtue of being generated from OCT data all OCTA flow signal acquires depth resolution through coherence gating. This idea can be written explicitly into Eq. [1] as

F 1 { I( k ) }( z )=2 r R I 0 n=1 N r S( n )γ[ 2z n ]  e i k 0 ( 2z n ) [4]

where rR and rS represent the reflectance of the reference and sample, respectively, I0 is the intensity of the illuminating beam, exp[ i k 0 ( 2z n ) ] represents the phase, and γ[ 2z n ] is the temporal autocorrelation accounting for coherence gating for a scattering event with path length (41). The summation over n accounts for the fact that the OCT signal is in general composed of N scattering events. The principle that enables depth resolution in OCT is that at any location the majority of these detected photons will be the result of backscattering from single scattering events, in which case the path length n is can be written n =2 z n , with the criterion for detection due to coherence gating that z n z . However, multiple scattering events can also contribute to the summation in Eq. [4] provided the total path length still sums to n 2 z n . Our experience with structural OCT informs us that the largest contribution to I is indeed from single backscattering events. In the context of OCTA specifically, it is worth noting that simulations indicate that red blood cells forward scatter light with relatively high probability (48,49). Such forward scattered photons can be subsequently backscattered. Forward scattering from moving red blood cells varies temporally, just as backscattering does. When photons forward-scattered by a red blood cells are subsequently back-scattered by deeper tissue they will contribute to flow signal detectable by OCTA.

The result of such multiple scattering events are projection artifacts. Arguably the most pernicious source of confusion in OCTA interpretation and analysis, they have the unfortunate capacity to mimic the appearance of real vasculature (Figure 1). Because they are largely the result of forward scattering, projection artifacts replicate the signal from superficial vasculature in deeper tissue. They are typically most prevalent near strongly back scattering tissue (such as the retinal pigment epithelium), since such material will reflect more of the forward-scattered photons that produce the artifacts. When this spurious signal appears in deeper vascular regions, for example when signal from the superficial vascular complex (SVC) is projected into the deep capillary plexus (DCP), it can reduce the accuracy of important OCTA measurements in such regions. Vessel density measurements from deeper plexuses made without projection artifact removal, as for instance in refs. (31,50-53), are not simply measurements from the plexus they were performed on, but some weighted amalgam of that plexus and more superficial vessels. Vessel density is not alone in this concern; any measurements extracted from vasculature in deeper layers will likewise conflate features from superficial plexuses and complexes. Other metrics, such as non-perfusion area, are disturbed even more by projection artifacts since artifactual flow signal can introduce signal into regions that are flow-free. These projection artifacts prevent us from detecting actual non-perfusion areas associated with diabetic retinopathy or retinal degeneration. Similarly, projection artifacts are especially problematic for detection and quantification of choroidal neovascularization (CNV) (Figure 2). As CNV erupts into the normally avascular outer retina, it could be detected by noticing flow in that region; however, projection artifacts can complicate this observation by introducing signal that must be separated from true flow in order to correctly ascertain the presence of CNV. Since the appearance of CNV is a major indicator in treatment of age-related macular degeneration (AMD) (13,54), accurate quantification of this development is extremely important from a clinical perspective; but at the same time, projection artifacts are often severe in the outer retina due to large amount of back scattered signal from the retinal pigment epithelium.

Figure 1 Comparison of retinal optical coherence tomographic angiography (OCTA) (3×3 mm2) from a healthy volunteer showing an uncorrected scan (row 1), shadow artifact removal from slab subtraction (row 2), and with projection resolution (PR-OCTA; row 3). Shown are merged structural and OCTA cross-sectional images (column 1) and en face OCTAs for the superficial vascular complex (SVC; column 2), intermediate capillary plexus (ICP; column 3), deep capillary plexus (DCP; column 4), and outer retina (column 5). The projection artifacts are especially prominent in the outer retina, which is avascular in healthy individuals (column 4, top row); the same flow pattern is also clearly visible in the ICP slab (column 2 top row). Projection artifacts also cause vessels in cross-section to appear as vertical strips (top left) rather than as the anatomically accurate dots as imaged by PR-OCTA. As can be seen from the cross-sectional image, while slab subtraction does remove projection artifacts from the en face images, it is not a three-dimensional technique, and so vessel tails are not removed. Slab subtraction also disrupts vessel continuity, as can be seen in the slab-subtracted ICP and DCP images. In the cross-sectional images, flow signal is colored coded according to location in the inner retina (violet), outer retina (yellow), and choroid (red). Slab definitions are SVC: from the nerve fiber layer to 67% of the way through the ganglion cell layer and inner plexiform layer; ICP: from the bottom of the SVC to midway through the inner nuclear layer; DCP: from the bottom of the ICP through the outer plexiform layer; outer retina: from the bottom of the outer plexiform layer to Bruch’s membrane. Collectively the SVC, ICP, and DCP form the inner retina.
Figure 2 Choroidal neovascularization as imaged by different methods for projection artifact removal. Cross-sections show flow signal (violet: inner retinal; yellow: outer retinal; red: choroidal flow) overlaid on structural optical coherence tomographic (OCT). (A1) original optical coherence tomographic angiography (OCTA) and (A2) projection-resolved OCTA (PR-OCTA). Artifactual tails that cause vessels to appear as vertical lines, rather than dots in cross-section, are clearly visible in the uncorrected image. (B) The superficial vascular complex slab. (C) Original OCTA angiogram of the outer retina. In a healthy eye, this slab is avascular, but choroidal neovascularization (CNV) is visible here, though heavily obscured by projection artifacts. The projection artifacts mimic the appearance of the superficial vasculature. (D) The same slab, after slab subtraction projection artifact removal. While the projection artifacts are removed, the vessels within the CNV lesion are disrupted, which may hinder some quantification. (E) The same slab viewed with PR-OCTA. While some residual projection artifacts are visible, the vessels in the CNV lesion remain continuous. Slab definitions are SVC: from the nerve fiber layer to 67% of the way through the ganglion cell layer and inner plexiform layer; ICP: from the bottom of the SVC to midway through the inner nuclear layer; DCP: from the bottom of the ICP through the outer plexiform layer; outer retina: from the bottom of the outer plexiform layer to Bruch’s membrane. Collectively the SVC, ICP, and DCP form the inner retina.

In addition to replicating superficial vascular features in deeper anatomic slabs, projection artifacts also produce “tails” on vessels in cross-sectional images (Figure 1). These tails can make determination of the depth of the CNV lesion problematic, and therefore infringe on our ability to determine CNV type. The artifactual tails also interfere substantially with visualization of retinal angiomatous proliferation, since they can mask real vessels connecting retinal and choroidal circulation as well as produce spurious signal that looks like such vessels (55).

One simple means of removing projection artifacts is slab subtraction (3,20,56). In this approach, the signal in superficial plexuses or complexes is subtracted, possibly after weighting, from angiograms in deeper tissue. This operation has the advantage of unambiguously removing projection artifacts, but also has several drawbacks. For one, real flow signal that happens to coincide with the projection artifact signal will also be removed. This means that slab subtraction cannot rescue measurements of vessel density in the DCP, since slab-subtracted angiograms would lead to underestimates in the same way un-modified angiograms with projection artifacts present would lead to over-estimates. Other OCTA metrics are similarly affected by this approach, with vessel connectivity in particular suffering. For this reason, slab subtraction is best applied for measurements performed in avascular regions. But even in such regions other measurements we might be interested in, for example CNV morphology, will be disrupted. An additional limitation is that slab subtraction operates exclusively on en face images. It cannot be used to remove the tails that projection artifacts introduce to vessels in cross-section. Finally, slab subtraction also requires accurate slab segmentation (from which to determine what signal should be removed from deeper layers). Correct slab segmentation is often difficult without intelligent algorithms, such as in (57,58), especially when eyes are affected by pathology—i.e., precisely when we typically employ OCTA in the first place. When slabs aren’t properly segmented, slab subtracted angiograms may incorporate flow signal removal that doesn’t even correspond to features from superficial layers.

A better approach for removing projection artifacts is projection-resolved OCTA (PR-OCTA) (59,60) (Figure 1). PR-OCTA algorithms are based on the observation that reflectance-normalized signal in projection artifacts is smaller than that of true in situ flow in overlying voxels in the same axial line. Specifically, the structural OCT signal is used to weigh the flow signal in OCTA volume, and the algorithm keeps the flow values at the successively higher peaks (in situ flow) along each A-line and suppress the rest to background level. Since this operation can be performed volumetrically, PR-OCTA has the additional capability of removing projection artifact tails from cross-sectional images. While PR-OCTA generally yields cleaner volumes that slab subtraction, it does still have some limitations. In low quality scans PR-OCTA can retain more residual artifacts than slab subtraction in the outer retina- however, PR-OCTA will not, like slab subtraction, disrupt the morphology of pathological vessels that may be present there. For this reason, it can be useful to rely on a suite of en face images including both PR-OCTA and slab subtraction to help more fully characterize projection artifacts, as in for example a recent automated choroidal neovascularization detection algorithm (17). PR-OCTA has also only been tested in an ophthalmological context. While the principles on which it relies should enable the technique to work in other tissues, this remains unverified.

Beyond these published approaches, commercial instruments now also include projection artifact removal algorithms. As these methods are proprietary, their specifics are not covered in this review. However, given that any OCTA image may contain residual artifacts, it is still important to inspect them.

The removal of projection artifacts allows retinal plexuses and complexes to be individually visualized (12). This capability enables more refined observation of pathological developments, and from PR-OCTA we now know that several diseases manifest differently in different plexuses (61). For instance, in glaucoma capillary loss primarily affects the superficial vascular complex, with relative sparing of deeper plexuses (26,62). The opposite is true in retinitis pigmentosa, where the deep capillary plexus is most severely affected (32,35,63,64). Even in diabetic retinopathy or vessel occlusions, in which all retinal plexuses suffer capillary drop out, the patterns of loss vary by plexus (37,65-67). PR-OCTA specifically is also particularly useful for imaging CNV, as it allows precise determination of lesion depth and can therefore distinguish CNV type (18,68,69); this is more difficult to achieve with projection removal techniques that do not operate volumetrically. Additionally, PR-OCTA can detect vascular connections between the choroidal and retinal circulations, and so is also advantageous for staging macular telangiectasia and retinal angiomatous proliferation (55,61,70).

Eye motion

Blood flow is not the only source of motion in the eye, and any motion contrast can produce OCTA signal. Such motion has several origins that can manifest differently.

Microsaccades occur every few seconds, which is the same time scale as a complete OCTA data acquisition. For this reason, they are often present in OCTA B-frames. They manifest as bright stripes in en face OCTA (Figure 3). Both hardware and software solutions can remove these stripes. Real-time tracking allows detection of microsaccides during OCTA acquisition; when microsaccades are detected instruments can rescan the affected location (72-76). Microsaccadic signal can be removed in software by omitting the affected scans (71,77). Instead, if multiple volumes are available, they can be registered and merged to recover the lost B-frames (77,78-81). This solution obviates the need for rescanning, and is often performed by scanning separately along each lateral scanning axis in the fast direction (i.e., x-fast and y-fast scanning schemes; Figure 3). Finally, real-time tracking of eye movement can be used to track and rescan areas affected by microsaccades when necessary (72-74). While this approach may increase the duration of imaging sessions some, it can be used to completely remove microsaccadic artifacts. Most tracking software relies on auxiliary technology (for instance, scanning laser ophthalmoscope), although a recent study has demonstrated real time tracking that relies solely on OCTA data (82).

Figure 3 x-fast and y-fast optical coherence tomographic angiography (OCTA) registration. Top row: OCT reflectance volumes completed in an (A) x-fast and (B) y-fast direction, and (C) the volume obtained by registering and merging these volumes (labeled MCT, for motion corrected technology). Bottom row: en face OCTA images corresponding to these volumes. In (D) and (E), the bright stripes are due to microsaccades; these artifacts are removed by the registration technique. Reprinted with permission from (71).

Ocular pulsation or drift are also sources of bulk motion. Like microsaccades, these are common disruptions in OCTA imaging, so they are a common issue in OCTA. The velocity due to this variety of bulk motion can be slow enough that it does not seriously jeopardize OCTA vessel contrast, but this is not always the case. Bulk motion of this variety produces phase noise, and so it is in particular a concern for complex- and phase-based OCTA processing. It is essential for these approaches that the phase noise due to bulk motion be removed before OCTA computation.

These approaches to compensating for bulk motion relied on the observation that in any given OCTA volume, most voxels will be avascular. The bulk motion contribution can then be reasonably estimated by considering statistics of the measured flow signal across all voxels. Several different approaches are possible based off of this observation; one of the earliest, due to Makita et al. with a phase-based OCTA method (83), constructed histograms of the phase-shift value between adjacent line-scans. They used the mode of this distribution as an estimate of the bulk motion contribution. An and Wang, in a complex-signal-based approach, instead used average phase change along a line-scan as the bulk motion contribution method (84). A subtlety for this approach is that a suitable threshold must be applied to remove phase noise, which averages to zero. Inclusion of phase noise in the bulk motion estimate, then, would tend to lead to underestimates. A similar problem occurs with phase wrapping. As OCTA transitioned from measuring motion contrast between subsequent line scans to motion contrast between subsequent cross-sectional scans, the time between scans increased, which had the incidental consequence of introducing a phase ambiguity into phase contrast measurements. This again leads to an underestimate since we can only measure phase within [−π, π]. A standard-deviation-based approach (43) or iterated averaging (85) can resolve this ambiguity and remove this contribution to bulk motion noise.

What all of these approaches have in common is that they appeal to a single test statistic (whether mean, mode, or standard deviation) in order to estimate the bulk motion contribution to the motion contrast signal. A more thorough approach at the post-processing level (after OCTA computation) can improve on these results by sampling more of the bulk motion statistics. One such approach is a regression-based approach that removes bulk motion-associated background by fitting flow signal statistics to an estimate of bulk motion signal strength derived from flow phantom experiments (Figure 4) (87). This approach achieves a better signal-to-noise ratio contrast than median subtraction approaches, and has shown utility in multiple instruments.

Figure 4 Bulk motion artifact removal on two different optical coherence tomographic (OCT) devices. Top row: AngioVue (OptoVue, USA). Bottom row: AngioPlex (Zeiss, Germany). The first column on the left shows the original en face projection of the superficial vascular complex (SVC). The remaining columns show binarization of the raw data (second column), binarization after median subtraction (third column), and after regression-based bulk motion subtraction (last column). Note the prominent horizontal motion artifacts incorporated into the vessel masks created from the original and median-subtracted images (columns 2 and 3) that are removed by the regression-based method (column 4). For slab definitions see the caption to Figure 1. Reprinted with permission from (86).

Signal attenuation

Signal strength in OCTA is tied to reflectance magnitude, whether directly (as in Eq. [2] or other reflectance/complex amplitude-based approaches) or indirectly (as in Eq. [3] or other correlation or ratio-based approaches).

Overall signal intensity in images is quantified on many commercial instruments (for instance signal strength index, SSI, from OptoVue). The signal intensity can influence OCTA metrics, and should be compensated for in order to prevent biased measurements (88). Loss of signal across an entire image is often caused by defocus, and is especially detrimental to OCTA measurements that rely on quantifying flow signal intensity (rather than vessel shape, for instance) (89). Vessel density, for example, can exhibit 10% variation within clinically acceptable scans when measurements are made on the same eye under different signal strength conditions (89). In addition to reducing contrast between vessels and background, defocus also broadens vessel width—another source of artificial variation in vessel density. While traditional, filter-based image processing techniques are sensitive to this variation, modern machine-learning-based quantification can avoid mismeasurement due to defocus (17,22). This is important since OCTA data sets often include defocused scans, but the degree of defocus may not always be evident. Beyond defocus, other common sources of global signal attenuation are cataracts and tear film break up. Since the tear film is responsible for some of the focusing power of the eye, its loss during imaging can lead to dimmer images.

In addition to global signal loss, local signal loss also occurs in OCTA imaging. This is often caused by severe signal attenuation beneath hyperreflective material along the beam path. Hyperreflective material may be found in the retina, for instance the hyperreflective foci sometimes observed in diabetic retinopathy (90,91). But shadowing can also be caused by material outside of the retina—for example, vitreous floaters. Vignetting (Figure 5) is also another common source of signal dropout. Depending on the degree of signal loss, it may be impossible to recover information like vessel density in a particular region of the OCTA image. When vessel density is calculated for an entire image, local signal loss will of course suppress the global value. However, local signal loss can appear similar to real capillary dropout while still reducing vessel density. This is a challenging issue for traditional hand-crafted image processing algorithms. Fortunately, modern deep-learning-based algorithms have demonstrated a capability to distinguish shadowing artifacts from real pathology (22) (Figure 5).

Figure 5 Automated avascular area detection. (A) Reflectance optical coherence tomographic (OCT) image, with shadow artifacts visible to the left. (B) Optical coherence tomographic angiography (OCTA) image of the superficial vascular complex (SVC), with some apparently avascular area actually caused by shadow artifacts. (C) Manually graded ground avascular area (green) overlaid on the angiogram. (D) Artificial intelligence produced avascular area (blue) and area identified as shadow artifact (yellow) by the algorithm. For slab definitions see the caption to Figure 1. Reprinted from Guo et al. (22).

Discussion and future directions

OCTA technology is still in its infancy, and for this reason it is unsurprising that images often exhibit artifacts. As the previous sections indicate, since its introduction we have collectively and iteratively developed better solutions for removing some of the most prominent artifacts. At the same time, these artifacts still exist and can affect OCTA data, even with state of the art removal techniques. Can we do better?

Even without significant investment in new technology we are likely to see reduction in some artifacts as OCTA components improve. Faster lasers, such as swept source, vertical-cavity surface-emitting laser that could be incorporated into new instruments are arriving without any effort on the part of the OCTA community. Increased scanning speeds will reduce bulk motion artifacts of their own accord, and also reduce the number of microsaccade line artifacts present in images by reducing the overall procedure time. However, acquisition speed ultimately competes with flow detection, since the dynamic range in OCTA systems is set in part by the time between B-scans, with shorter inter-frame times becoming decreasingly sensitive to slow flow (92). Instead, real-time tracking could be improved to remove bulk motion artifacts. Here the limiting step is GPU processing or data transfer speeds. Current state-of-the-art research systems can process OCTA in real time at megahertz scan rates (93-95). With better GPUs, swifter tracking will be able to suppress more bulk motion artifacts.

Incorporating these better components into a new generation of OCTA devices can help reduce motion artifacts. By augmenting OCTA instruments with adaptive optics (96) projection artifacts can also be suppressed. Adaptive optics systems can be used to compensate for aberrations in the eye, increasing system optical resolution and enabling visualization of capillaries at anatomic caliber (rather than the expanded caliber seen in current OCTA systems) (97,98). This has the added benefit of removing projection artifacts, since axial resolution can also be improved with this approach. However, state-of-the-art research instruments that incorporate this technology currently employ reduced fields-of-view in order to keep total imaging times feasible—a limitation that will need to be overcome before they become widely applicable.

Both adaptive optics and improved OCTA acquisition and tracking are means to reduce the number of artifacts in images through hardware. Improvements in software also have the potential to clean artifacts better. Modern deep-learning-based image reconstruction has demonstrated the capability to improve signal-to-noise ratios when generating OCTA data from the measured complex signal (99). In Liu et al., this was achieved by training a network on OCTA generated from 48 consecutive B-frames, and requires 4 input B-frames to perform the reconstruction (100). Lee et al. instead reconstruct OCTA from single B-frames, which has the innate advantage of suppressing motion artifacts due to the abbreviated times required to acquire one (rather the usual two) data volume (101). In principle, both of these networks could have been trained on OCTA images in which artifacts were already suppressed, so that artifact suppression could be learned simultaneously. However, deep learning techniques such as these could introduce new artifacts of their own—Lee et al. noted that some capillaries were not correctly displayed by their network, while Liu et al. noted that some vessels gained an artifactual “wave” pattern. It is possible that other, more subtle artifacts that go unnoticed by researchers may also be introduced by these approaches.

Deep-learning based image reconstruction is a means by which artifacts in images can be removed; an alternative is to attempt to accurately quantify OCTA data in which artifacts are present (22). Again, deep-learning methods have shown success in this respect—as noted above, networks can learn features that allow them to identify shadow artifacts.

None of the approaches discussed here can alleviate the detrimental effect of artifacts in OCTA on their own, but neither are any mutually exclusive. Future devices incorporating all or some of these advantages will likely be marred by substantially fewer artifacts that current images.


Even if artifacts do still exist in the current state-of-the-art in OCTA, we have made large strides in cleaning them from images and extracting more accurate measurements from images in which they exist. Clinicians and researchers that are aware of how artifacts affect OCTA will best be able to take advantage of this powerful technology.


Funding: This work was supported by grants R01EY027833, R01 EY024544, R01 EY023285, R01 EY031394, P30 EY010572 from the National Institutes of Health (Bethesda, MD) and an unrestricted departmental funding grant and William & Mary Greve Special Scholar Award from Research to Prevent Blindness (New York, NY).


Provenance and Peer Review: With the arrangement by the Guest Editors and the editorial office, this article has been reviewed by external peers.

Conflicts of Interest: All authors have completed the ICMJE uniform disclosure form (available at The special issue “Advanced Optical Imaging in Biomedicine” was commissioned by the editorial office without any funding or sponsorship. DH reports grants from NIH, grants, stock ownership, and personal fees from Optovue, Inc., outside the submitted work. YJ reports grants from NIH, grants and personal fees from Optovue, Inc., grants from RPB foundation, outside the submitted work. These potential conflicts of interest have been reviewed and managed by OHSU. The authors have no other conflicts of interest to declare.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See:


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Cite this article as: Hormel TT, Huang D, Jia Y. Artifacts and artifact removal in optical coherence tomographic angiography. Quant Imaging Med Surg 2021;11(3):1120-1133. doi: 10.21037/qims-20-730