@article{QIMS29392,
author = {Lin Yang and Xiao Yu and Ashley M. Fuller and Melissa A. Troester and Amy L. Oldenburg},
title = {Characterizing optical coherence tomography speckle fluctuation spectra of mammary organoids during suppression of intracellular motility},
journal = {Quantitative Imaging in Medicine and Surgery},
volume = {10},
number = {1},
year = {2019},
keywords = {},
abstract = {Background: An understanding of how the mammary gland responds to toxicant and drug exposures can shed light on mechanisms of breast cancer initiation/progression and therapeutic effectiveness, respectively. In this study, we employed noninvasive, label-free and high-throughput optical coherence tomography speckle fluctuation spectroscopy (OCT-SFS) to track exposure-response relationships in three-dimensional (3D) mammary epithelial organoid models.
Methods: OCT-SFS is sensitive to relatively high speed (~0.16–8 µm/min) motions of subcellular light scattering components occurring over short (~2–114 s) time scales, termed “intracellular motility.” In this study, OCT speckle fluctuation spectra are quantified by two metrics: the intracellular motility amplitude, M, and frequency-dependent motility roll-off, α. OCT-SFS was performed on human mammary organoid models comprised of pre-malignant MCF10DCIS.com cells or MCF7 adenocarcinoma cells over 6 days of exposure to either a microtubule inhibitor (Paclitaxel, Taxol) or a myosin II inhibitor (Blebbistatin). Raw values of α and M were normalized to a dynamic range corresponding to fixed (0%) and live/homeostatic (100%) organoids for each cell line.
Results: In this work, we observed a significant decrease in both M and α of MCF10DCIS.com organoids after 24 hours of exposure to Taxol (P},
issn = {2223-4306}, url = {https://qims.amegroups.org/article/view/29392}
}