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Intravital assessment of angioarchitecture in rat hepatocellular nodules using in vivo fluorescent microscopy

  
@article{QIMS26547,
	author = {Yi Liu and Tao Lu and Congcong Wang and Hui Li and Ke Xu and Peiling Li},
	title = {Intravital assessment of angioarchitecture in rat hepatocellular nodules using  in vivo  fluorescent microscopy},
	journal = {Quantitative Imaging in Medicine and Surgery},
	volume = {9},
	number = {6},
	year = {2019},
	keywords = {},
	abstract = {Background: To prospectively evaluate the stepwise changes that occur in intra-nodular microvessels and microcirculation during the carcinogenesis process of hepatocellular nodules by using in vivo fluorescent microscopy, and to compare these with pathological changes.
Methods: Forty-five 10-week-old male Wistar rats received drinking water containing N-nitrosomorpholine at 10 mg/100 mL for 18weeks to develop multiple hepatocellular carcinomas (HCC) and dysplastic nodules (DN) in the liver; meanwhile, the non-lesion liver tissues become fibrotic. The microvascular morphological change and hemodynamic change of two lesion areas (HCC or DN) and one non-lesion area in each rat were observed with in vivo fluorescent microscope. After in vivo microscopy, 90 nodules and 45 non-lesion liver tissues that were observed were removed for pathological study. The microvessel density (MVD), branch density (BD), and cell density (CD) of these lesions were compared with the Kruskal-Wallis test and Mann-Whitney test, with an overall statistical significance of 0.05.
Results: The intra-nodular microvessels appeared tortuous, with irregular branching and abrupt diameter changes to form irregular convoluted networks in the HCC. This was distinctly different from the appearance of DN and non-lesion liver parenchyma. The MVD and BD of HCC were less than that of the DN and non-lesion liver parenchyma (P},
	url = {http://qims.amegroups.com/article/view/26547}
}